ARG66617

anti-AIM2 antibody

anti-AIM2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes AIM2
反应物种 Hu
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 AIM2
抗原物种 Human
抗原 KLH-conjugated synthetic peptide within the center region of Human AIM2.
偶联标记 Un-conjugated
別名 PYHIN4; Interferon-inducible protein AIM2; Absent in melanoma 2

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:100 - 1:500
IHC-P1:100 - 1:200
WB1:500 - 1:1000
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 39 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
抗菌剂 0.01% Sodium azide
稳定剂 30% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 9447 Human AIM2

Swiss-port # O14862 Human Interferon-inducible protein AIM2

基因名称 AIM2
全名 absent in melanoma 2
背景介绍 AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq, Jul 2008]
生物功能 Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. [UniProt]
细胞定位 Nucleus. Cytoplasm. Note=Activated inflammasomes can aggregate in the cytosol as speck-like particles. [UniProt]
预测分子量 39 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG66617 anti-AIM2 antibody ICC/IF image

    Immunofluorescence: Formalin-fixed Jurkat cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66617 anti-AIM2 antibody (red) in 3% BSA-PBS and incubated overnight at 4°C. DAPI (blue) for nuclear staining.

  • ARG66617 anti-AIM2 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung cancer tissue section. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66617 anti-AIM2 antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • ARG66617 anti-AIM2 antibody WB image

    Western blot: Jurkat whole cell lysate stained with ARG66617 anti-AIM2 antibody.