ARG54374

anti-AIF (AIFM1) antibody

anti-AIF (AIFM1) antibody for ICC/IF,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody

概述

产品描述 Rabbit Polyclonal antibody recognizes AIF (AIFM1)
反应物种 Hu
应用 ICC/IF, WB
特异性 This antibody recognizes human AIF (AIFM1)(67 kDa).
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 AIF (AIFM1)
抗原物种 Human
抗原 Peptide corresponding to aa 109-122 at the N-terminus of human AIF.
偶联标记 Un-conjugated
別名 CMTX4; NAMSD; COWCK; Apoptosis-inducing factor 1, mitochondrial; CMT2D; EC 1.1.1.-; NADMR; PDCD8; COXPD6; AIF; Programmed cell death protein 8

应用说明

应用建议
应用 推荐稀释比
ICC/IF20 μg/mL
WB1-2 μg/mL
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 K562 and Jurkat

属性

形式 Liquid
纯化 Immunoaffinity chroma-tography
缓冲液 PBS (pH 7.4) and 0.02% Sodium azide
抗菌剂 0.02% Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 9131 Human AIFM1

Swiss-port # O95831 Human Apoptosis-inducing factor 1, mitochondrial

基因名称 AIFM1
全名 apoptosis-inducing factor, mitochondrion-associated, 1
背景介绍 A novel protein that causes chromatin condensation and DNA fragmentation has been designated Apoptosis Inducing Factor (AIF). AIF localizes in mitochondria and translocates to the nucleus when apoptosis is induced. This event is followed by the release of chytochrome c and caspase-9 from mitochondria. AIF is highly conserved between human and mouse and widely expressed in different cell types and tissues.
生物功能 Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. [UniProt]
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
预测分子量 67 kDa
翻译后修饰 Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation. Ubiquitination at Lys-255 by XIAP/BIRC4 blocks its ability to bind DNA and induce chromatin degradation, thereby inhibiting its ability to induce cell death.

检测图片 (2) Click the Picture to Zoom In

  • ARG54374 anti-AIF (AIFM1) antibody WB image

    Western Blot: K562 stained with ARG54374 anti-AIF (AIFM1) antibody at 1 μg/ml dilution.

  • ARG54374 anti-AIF (AIFM1) antibody ICC/IF image

    Immunocytochemistry: K562 stained with ARG54374 anti-AIF (AIFM1)antibody at 20 μg/ml dilution.