ARG62667
anti-AHNAK1 antibody [EM-09]
anti-AHNAK1 antibody [EM-09] for ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [EM-09] recognizes AHNAK1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-Fr, IP, WB |
| 特异性 | The clone EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | EM-09 |
| 同位型 | IgG1 |
| 靶点名称 | AHNAK1 |
| 抗原物种 | Human |
| 抗原 | Bacterially expressed fragment of N-terminal domain of human AHNAK1. |
| 偶联标记 | Un-conjugated |
| 別名 | Desmoyokin; AHNAKRS; Neuroblast differentiation-associated protein AHNAK |
应用说明
| 应用建议 |
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| 应用说明 | ICC/IF: Permeabilization is required. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | WB and ICC/IF: HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q09666 Human Neuroblast differentiation-associated protein AHNAK |
|---|---|
| 基因名称 | AHNAK |
| 全名 | AHNAK nucleoprotein |
| 背景介绍 | AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex. |
| 生物功能 | May be required for neuronal cell differentiation. [UniProt] |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 629 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG62667 anti-AHNAK1 antibody [EM-09] ICC/IF image
Immunofluorescence: Human primary fibroblasts stained with ARG62667 anti-AHNAK1 antibody [EM-09] (green)
Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue). -
ARG62667 anti-AHNAK1 antibody [EM-09] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG62667 anti-AHNAK1 antibody [EM-09] (red)
Cell nuclei was stained with DAPI (blue). -
ARG62667 anti-AHNAK1 antibody [EM-09] IHC-Fr image
Immunohistochemistry: Murine tongue section stained with ARG62667 anti-AHNAK1 antibody [EM-09].
