ARG45761
anti-AGO3 / Eif2c3 antibody
anti-AGO3 / Eif2c3 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes AGO3 / Eif2c3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AGO3 / Eif2c3 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human AGO3 / Eif2c3. |
| 偶联标记 | Un-conjugated |
| 別名 | eIF2C 3; EIF2C3; Argonaute RISC catalytic component 3; Argonaute3; Protein argonaute-3; hAgo3; Eukaryotic translation initiation factor 2C 3; eIF-2C 3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 97 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AGO3 |
| 全名 | argonaute RISC catalytic component 3 |
| 背景介绍 | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. [UniProt] |
| 细胞定位 | Cytoplasm, P-body. [UniProt] |
| 预测分子量 | 97 kDa |
| 翻译后修饰 | Acetylation; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
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ARG45761 anti-AGO3 / Eif2c3 antibody IHC-P image
Immunohistochemistry: Human cervical carcinoma stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 2 μg/ml dilution.
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ARG45761 anti-AGO3 / Eif2c3 antibody ICC/IF image
Immunofluorescence: U2OS stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 5 μg/ml dilution.
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ARG45761 anti-AGO3 / Eif2c3 antibody WB image
Western blot: K562 stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 0.5 μg/ml dilution.
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ARG45761 anti-AGO3 / Eif2c3 antibody FACS image
Flow Cytometry: Jurkat stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 1 µg/10^6 cells dilution.
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ARG45761 anti-AGO3 / Eif2c3 antibody WB image
Western blot: Rat brain stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 0.5 μg/ml dilution.
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ARG45761 anti-AGO3 / Eif2c3 antibody WB image
Western blot: Mouse brain stained with ARG45761 anti-AGO3 / Eif2c3 antibody at 0.5 μg/ml dilution.
