ARG45645
anti-AFF4 antibody
anti-AFF4 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes AFF4 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AFF4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to N-terminal region of human AFF4. |
| 偶联标记 | Un-conjugated |
| 別名 | AFF4; AF4/FMR2 family, member 4; AF4/FMR2 family member 4; Protein AF-5q31; CHOPS; MCEF; ALL1-fused gene from chromosome 5q31 protein; Major CDK9 elongation factor-associated protein; AF5Q31 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 150 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AFF4 |
| 全名 | AF4/FMR2 family, member 4 |
| 背景介绍 | The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011] |
| 生物功能 | Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 127 kDa |
| 翻译后修饰 | Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG45645 anti-AFF4 antibody IHC-P image
Immunohistochemistry: Human placenta stained with ARG45645 anti-AFF4 antibody at 2 μg/ml dilution.
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ARG45645 anti-AFF4 antibody WB image
Western blot: Hela stained with ARG45645 anti-AFF4 antibody at 0.5 μg/ml dilution.
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ARG45645 anti-AFF4 antibody FACS image
Flow Cytometry: SiHa stained with ARG45645 anti-AFF4 antibody at 1 µg/10^6 cells dilution.
