ARG57109

anti-ADK antibody [4F8]

anti-ADK antibody [4F8] for Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Mouse Monoclonal antibody [4F8] recognizes ADK
反应物种 Hu
应用 FACS, ICC/IF, WB
宿主 Mouse
克隆 Monoclonal
克隆号 4F8
同位型 IgG1, kappa
靶点名称 ADK
抗原物种 Human
抗原 Recombinant fragment around aa. 22-362 of Human ADK
偶联标记 Un-conjugated
別名 Adenosine kinase; AK; Adenosine 5'-phosphotransferase; EC 2.7.1.20

应用说明

应用建议
应用 推荐稀释比
FACSAssay-dependent
ICC/IFAssay-dependent
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 10% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 132 Human ADK

Swiss-port # P55263 Human Adenosine kinase

基因名称 ADK
全名 adenosine kinase
背景介绍 This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
生物功能 ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. [UniProt]
预测分子量 41 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG57109 anti-ADK antibody [4F8] ICC/IF image

    Immunofluorescence: A549 cells line stained with ARG57109 anti-ADK antibody [4F8] at 1:100 (Green).

    DAPI (Blue) for nucleus staining.

  • ARG57109 anti-ADK antibody [4F8] FACS image

    Flow Cytometry: A549 cell line stained with ARG57109 anti-ADK antibody [4F8] at 2- 5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).

  • ARG57109 anti-ADK antibody [4F8] WB image

    Western blot: 35 µg of HepG2 and 293T cell lysates stained with ARG57109 anti-ADK antibody [4F8] at 1:1000.