ARG66528
anti-ADD3 / gamma Adducin antibody
anti-ADD3 / gamma Adducin antibody for Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ADD3 / gamma Adducin |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ADD3 / gamma Adducin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 400-480 of Human ADD3 / gamma Adducin. |
| 偶联标记 | Un-conjugated |
| 別名 | Gamma-adducin; ADDL; Adducin-like protein 70 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | 75 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ADD3 |
| 全名 | adducin 3 (gamma) |
| 背景介绍 | Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq, Jul 2008] |
| 生物功能 | Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. [UniProt] |
| 预测分子量 | 79 kDa |
| 翻译后修饰 | Sumoylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66528 anti-ADD3 / gamma Adducin antibody WB image
Western blot: 3T3 cell lysate stained with ARG66528 anti-ADD3 / gamma Adducin antibody.
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ARG66528 anti-ADD3 / gamma Adducin antibody WB image
Western blot: 293 cell lysate stained with ARG66528 anti-ADD3 / gamma Adducin antibody.
