ARG59548
anti-ADAMTS13 antibody
anti-ADAMTS13 antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ADAMTS13 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ADAMTS13 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to A299-R488 of Human ADAMTS13. |
| 偶联标记 | Un-conjugated |
| 別名 | von Willebrand factor-cleaving protease; vWF-CP; EC 3.4.24.87; ADAM-TS 13; VWFCP; A disintegrin and metalloproteinase with thrombospondin motifs 13; ADAM-TS13; vWF-cleaving protease; ADAMTS-13; C9orf8 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q769J6 Mouse A disintegrin and metalloproteinase with thrombospondin motifs 13 Swiss-port # Q76LX8 Human A disintegrin and metalloproteinase with thrombospondin motifs 13 |
|---|---|
| 基因名称 | ADAMTS13 |
| 全名 | ADAM metallopeptidase with thrombospondin type 1 motif, 13 |
| 背景介绍 | This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| 生物功能 | Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. [UniProt] |
| 细胞定位 | Secreted. Note=Secretion enhanced by O-fucosylation of TSP type-1 repeats. [UniProt] |
| 预测分子量 | 154 kDa |
| 翻译后修饰 | Glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS13. May also be C-glycosylated on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and also N-glycosylated. These other glycosylations can also facilitate secretion. The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG59548 anti-ADAMTS13 antibody WB image
Western blot: 50 µg of HepG2 whole cell lysate stained with ARG59548 anti-ADAMTS13 antibody at 0.5 µg/ml dilution, under non-reducing (left) or reducing (right) conditions.
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ARG59548 anti-ADAMTS13 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Mouse liver and Rat brain lysates stained with ARG59548 anti-ADAMTS13 antibody at 0.5 µg/ml dilution, overnight at 4°C.
