ARG43632
anti-ADAM9 antibody
anti-ADAM9 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit polyclonal antibody recognizes ADAM9 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ADAM9 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment corresponding to a.a. R212-D247 of Human ADAM9. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Disintegrin and metalloproteinase domain-containing protein 9 |
| 別名 | MDC9; Meltrin-gamma; ADAM 9; Metalloprotease/disintegrin/cysteine-rich protein 9; Disintegrin and metalloproteinase domain-containing protein 9; Cellular disintegrin-related protein; EC 3.4.24.-; CORD9; Mltng; MCMP; Myeloma cell metalloproteinase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | A431, U87-MG | ||||||||
| 实际分子量 | ~ 70-80 kDa (mature form), 100-115 kDa (pro-form) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13443 Human Disintegrin and metalloproteinase domain-containing protein 9 |
|---|---|
| 基因名称 | ADAM9 |
| 全名 | ADAM metallopeptidase domain 9 |
| 背景介绍 | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010] |
| 生物功能 | Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP. [UniProt] |
| 细胞定位 | Cell membrane, Membrane, Secreted |
| 预测分子量 | 90.5 kDa |
| 翻译后修饰 | Proteolytically cleaved in the trans-Golgi network before it reaches the plasma membrane to generate a mature protein. The removal of the pro-domain occurs via cleavage at two different sites. Processed most likely by a pro-protein convertase such as furin, at the boundary between the pro-domain and the catalytic domain. An additional upstream cleavage pro-protein convertase site (Arg-56/Glu-57) has an important role in the activation of ADAM9. Phosphorylation is induced in vitro by phorbol-12-myristate-13-acetate (PMA). [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG43632 anti-ADAM9 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human uterus tissue stained with ARG43632 anti-ADAM9 antibody at 1:100 dilution. Antigen Retrieval: Heat mediated was performed using EDTA buffer (pH 8.0).
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ARG43632 anti-ADAM9 antibody ICC/IF image
Immunofluorescence: U20S cells were blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG43632 anti-ADAM9 antibody at 1:100 and for 30 minutes at 37°C.
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ARG43632 anti-ADAM9 antibody WB image
Western blot: A431 cell lysates stained with ARG43632 anti-ADAM9 antibody at 1:1500 dilution.
