ARG63476
anti-ADAM17 / TACE antibody
anti-ADAM17 / TACE antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes ADAM17 / TACE |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise isoform 1 (represented by NP_003174) and not isoform 2 (represented by NP_068604). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ADAM17 / TACE |
| 抗原物种 | Human |
| 抗原 | LQRQNRVDSKETEC |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD156b; TNF-alpha convertase; NISBD; ADAM 17; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease; TACE; NISBD1; ADAM18; EC 3.4.24.86; CSVP; CD156B; TNF-alpha-converting enzyme |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P78536 Human Disintegrin and metalloproteinase domain-containing protein 17 |
|---|---|
| 背景介绍 | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 93 kDa |
| 翻译后修饰 | The precursor is cleaved by a furin endopeptidase. Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. Phosphorylation at THR-735 by MAPK14 is required for ADAM17-mediated ectodomain shedding. |
检测图片 (4) Click the Picture to Zoom In
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ARG63476 anti-ADAM17 / TACE antibody WB image
Western Blot: Human Testis lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63476 anti-ADAM17 / TACE antibody at 0.2 µg/ml dilution.
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ARG63476 anti-ADAM17 / TACE antibody WB image
Western Blot: Daudi (A), Jurkat (B) and HeLa (C) lysates (35 µg total protein in RIPA buffer) stained with ARG63476 anti-ADAM17 / TACE antibody at 0.3 µg/ml dilution.
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ARG63476 anti-ADAM17 / TACE antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skin tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63476 anti-ADAM17 / TACE antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63476 anti-ADAM17 / TACE antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63476 anti-ADAM17 / TACE antibody at 3.75 µg/ml dilution followed by AP-staining.
