ARG43454
anti-ADAM17 / TACE antibody
anti-ADAM17 / TACE antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ADAM17 / TACE |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | This antibody recognizes human, mouse, and rat TACE, and 80-130kDa bands are detected in immunoblots. These bands may represent mature protein, precursor, and glycosylated TACE. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ADAM17 / TACE |
| 抗原物种 | Human |
| 抗原 | A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human ADAM17 / TACE (NP_003174.3). |
| 偶联标记 | Un-conjugated |
| Protein Full name | Disintegrin and metalloproteinase domain-containing protein 17 |
| 別名 | CD antigen CD156b; TNF-alpha convertase; NISBD; ADAM 17; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease; TACE; NISBD1; ADAM18; EC 3.4.24.86; CSVP; CD156B; TNF-alpha-converting enzyme |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HepG2 and mouse heart | ||||||||
| 实际分子量 | ~110 kDa (full length), ~90 kda (mature) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ADAM17 |
| 全名 | ADAM metallopeptidase domain 17 |
| 背景介绍 | TNF is synthesized as a 26kDa type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17kDa mature TNF . TNF converting enzyme (TACE) has been identified, and human and mouse TACE cDNAs have been cloned. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGF , L-selectin, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and mouse tissues. |
| 生物功能 | Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 93 kDa (isoform 1) |
| 翻译后修饰 | The precursor is cleaved by a furin endopeptidase. Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. Phosphorylation at THR-735 by MAPK14 is required for ADAM17-mediated ectodomain shedding. |
检测图片 (2) Click the Picture to Zoom In
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ARG43454 anti-ADAM17 / TACE antibody WB image
Western blot: 25 µg of HepG2 cell lysate stained with ARG43454 anti-ADAM17 / TACE antibody at 1:1000 dilution.
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ARG43454 anti-ADAM17 / TACE antibody WB image
Western blot: 25 µg of Mouse heart lysate stained with ARG43454 anti-ADAM17 / TACE antibody at 1:1000 dilution.
