ARG54334
anti-ADAM10 / KUZ / MADM antibody
anti-ADAM10 / KUZ / MADM antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ADAM10 / KUZ / MADM |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | This antibody recognizes human, mouse, and rat ADAM10. A band of 85kDa is detected in immunoblots and may represent precursor. Lower mw bands are detected in some cell lines, including Jurkat, and may represent the processed mature protein. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ADAM10 / KUZ / MADM |
| 抗原物种 | Human |
| 抗原 | Peptide corresponding to aa 732-748 of human ADAM10. This sequence is identical to those of bovine and rat and differs from mouse by one amino acid |
| 偶联标记 | Un-conjugated |
| 別名 | Kuzbanian protein homolog; kuz; HsT18717; MADM; RAK; EC 3.4.24.81; ADAM 10; AD18; CDw156; Mammalian disintegrin-metalloprotease; CD antigen CD156c; AD10; Disintegrin and metalloproteinase domain-containing protein 10; CD156c |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Jurkat and K562 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A purified. |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O14672 Human Disintegrin and metalloproteinase domain-containing protein 10 Swiss-port # O35598 Mouse Disintegrin and metalloproteinase domain-containing protein 10 |
|---|---|
| 基因名称 | ADAM10 |
| 全名 | ADAM metallopeptidase domain 10 |
| 背景介绍 | TNF , Notch and its ligand delta are all membrane-bound molecules which are cleaved by proteases to release mature proteins or functional receptors. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease) was recently found to cleave TNF , Notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 have been cloned and designated ADAM10, Kuzbanian (KUZ), and MADM, respectively. These genes are expressed in a variety of human and bovine tissues. |
| 生物功能 | Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody |
| 预测分子量 | 84 kDa |
| 翻译后修饰 | The precursor is cleaved by a furin endopeptidase. |
检测图片 (3) Click the Picture to Zoom In
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ARG54334 anti-ADAM10 / KUZ / MADM antibody IHC image
Immunofluorescence: Mouse Testis stained with ARG54334 anti-ADAM10 / KUZ / MADM antibody at 20 μg/ml dilution.
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ARG54334 anti-ADAM10 / KUZ / MADM antibody WB image
Western blot: Jurkat stained with ARG54334 anti-ADAM10 / KUZ / MADM antibody at 1 μg/ml dilution.
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ARG54334 anti-ADAM10 / KUZ / MADM antibody ICC/IF image
Immunofluorescence: K562 stained with ARG54334 anti-ADAM10 / KUZ / MADM antibody at 20 μg/ml dilution.
