ARG57036
anti-ACY1 / Aminoacylase 1 antibody [1E2]
anti-ACY1 / Aminoacylase 1 antibody [1E2] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1E2] recognizes ACY1 / Aminoacylase 1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1E2 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | ACY1 / Aminoacylase 1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-408 of Human Aminoacylase 1. |
| 偶联标记 | Un-conjugated |
| 別名 | ACY-1; N-acyl-L-amino-acid amidohydrolase; ACY1D; EC 3.5.1.14; HEL-S-5; Aminoacylase-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACY1 |
| 全名 | aminoacylase 1 |
| 背景介绍 | This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Nov 2010] |
| 生物功能 | Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). [UniProt] |
| 预测分子量 | 46 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] FACS image
Flow Cytometry: Hep3B cells stained with ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] at 2-5 µg/10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] WB image
Western blot: 40 µg of Jurkat, HepG2, HeLa, and A549 stained with ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] at 1:500.
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ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] WB image
Western blot: 50 ng of Recombinant Human ACY1 stained with ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] at 1:1000.
