ARG63492

anti-ACTR1B / ARP1B antibody

anti-ACTR1B / ARP1B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Signaling Transduction antibody

概述

产品描述 Goat Polyclonal antibody recognizes ACTR1B / ARP1B
反应物种 Hu
预测物种 Ms, Rat, Cow, Dog
应用 IHC-P, WB
特异性 This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 ACTR1B / ARP1B
抗原物种 Human
抗原 C-YEEDGSRAIHRKT
偶联标记 Un-conjugated
別名 PC3; Actin-related protein 1B; CTRN2; Beta-centractin; ARP1B

应用说明

应用建议
应用 推荐稀释比
IHC-P3 - 5 µg/ml
WB0.5 - 2 µg/ml
应用说明 IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 10120 Human ACTR1B

Swiss-port # P42025 Human Beta-centractin

背景介绍 This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq, Aug 2008]
研究领域 Signaling Transduction antibody
预测分子量 42 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG63492 anti-ACTR1B / ARP1B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human uterus tissue. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63492 anti-ACTR1B / ARP1B antibody at 3.8 µg/ml dilution followed by AP-staining.

  • ARG63492 anti-ACTR1B / ARP1B antibody WB image

    Western blot: Human heart lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63492 anti-ACTR1B / ARP1B antibody at 1 µg/ml dilution.