ARG57247
anti-ACTC1 antibody [RM257]
anti-ACTC1 antibody [RM257] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Monoclonal antibody [RM257] recognizes ACTC1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Bov, Rat |
| 应用 | IHC-P, WB |
| 特异性 | The antibody reacts to Human and Mouse alpha cardiac actin (Actin, alpha cardiac muscle 1). This antibody may also react to Bovine or Rat alpha-cardiac actin, as predicted by immunogen homology. |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | RM257 |
| 同位型 | IgG |
| 靶点名称 | ACTC1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the N-terminus of Human ACTC1. |
| 偶联标记 | Un-conjugated |
| 別名 | CMH11; CMD1R; ASD5; Actin, alpha cardiac muscle 1; LVNC4; Alpha-cardiac actin; ACTC |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.09% Sodium azide, 50% Glycerol and 1% BSA. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol and 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACTC1 |
| 全名 | actin, alpha, cardiac muscle 1 |
| 背景介绍 | Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008] |
| 生物功能 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt] |
| 预测分子量 | 42 kDa |
| 翻译后修饰 | Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
检测图片 (2) Click the Picture to Zoom In
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ARG57247 anti-ACTC1 antibody [RM257] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human heart tissue sections stained with ARG57247 anti-ACTC1 antibody [RM257] at 1:1000 dilution.
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ARG57247 anti-ACTC1 antibody [RM257] WB image
Western blot: Mouse heart tissue lysate stained with ARG57247 anti-ACTC1 antibody [RM257] at 1:1000 dilution.
