ARG66506
anti-ACPP antibody
anti-ACPP antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes ACPP |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1, kappa |
| 靶点名称 | ACPP |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human ACPP. |
| 偶联标记 | Un-conjugated |
| 別名 | PAP; ACP-3; TMPase; 5'-nucleotidase; ACP3; EC 3.1.3.5; Ecto-5'-nucleotidase; Thiamine monophosphatase; EC 3.1.3.2; 5'-NT; Prostatic acid phosphatase; PSAP |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Tris/EDTA buffer (pH 8.0) was used. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACPP |
| 全名 | acid phosphatase, prostate |
| 背景介绍 | ACPP is an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008] |
| 生物功能 | ACPP is a non-specific tyrosine phosphatase. It dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Isoform 2: the cellular form also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. Acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. [UniProt] |
| 细胞定位 | Isoform 1: Secreted. Isoform 2: Cell membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=Appears to shuttle between the cell membrane and intracellular vesicles. Colocalizes with FLOT1 at cell membrane and in intracellular vesicles (PubMed:17638863). Colocalizes with LAMP2 on the lysosome membrane (PubMed:17897319). [UniProt] |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection) which entrap HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG66506 anti-ACPP antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human benign prostatic hyperplasia stained with ARG66506 anti-ACPP antibody at 1:200 (4°C, overnight). Antigen Retrieval: Tris/EDTA buffer (pH 8.0) was used.
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ARG66506 anti-ACPP antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate stained with ARG66506 anti-ACPP antibody at 1:200 (4°C, overnight). Antigen Retrieval: Tris/EDTA buffer (pH 8.0) was used.
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ARG66506 anti-ACPP antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostatic carcinoma stained with ARG66506 anti-ACPP antibody at 1:200 (4°C, overnight). Antigen Retrieval: Tris/EDTA buffer (pH 8.0) was used.
