ARG65247
anti-ACPP antibody
anti-ACPP antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes ACPP |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ACPP |
| 抗原物种 | Human |
| 抗原 | C-YGIHKQKEKSRLQ |
| 偶联标记 | Un-conjugated |
| 別名 | PAP; ACP-3; TMPase; 5'-nucleotidase; ACP3; EC 3.1.3.5; Ecto-5'-nucleotidase; Thiamine monophosphatase; EC 3.1.3.2; 5'-NT; Prostatic acid phosphatase |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Human prostate | ||||||||
| 实际分子量 | ~ 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | ACPP is an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008] |
| 生物功能 | ACPP is a non-specific tyrosine phosphatase. It dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Isoform 2: the cellular form also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. Acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection) which entrap HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection. |
检测图片 (2) Click the Picture to Zoom In
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ARG65247 anti-ACPP antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65247 anti-ACPP antibody at 3.8 µg/ml dilution, followed by AP-staining.
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ARG65247 anti-ACPP antibody WB image
Western blot: 35 µg of Human prostate lysate (in RIPA buffer) stained with ARG65247 anti-ACPP antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
