ARG57045
anti-ACOT8 antibody [4D10]
anti-ACOT8 antibody [4D10] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [4D10] recognizes ACOT8 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4D10 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | ACOT8 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-319 of Human ACOT8. |
| 偶联标记 | Un-conjugated |
| 別名 | hACTE-III; hTE; Choloyl-coenzyme A thioesterase; EC 3.1.2.27; Peroxisomal acyl-coenzyme A thioester hydrolase 1; HNAACTE; PTE1; PTE2; Peroxisomal long-chain acyl-CoA thioesterase 1; PTE-2; PTE-1; Thioesterase II; hACTEIII; HIV-Nef-associated acyl-CoA thioesterase; Acyl-CoA thioesterase 8; Acyl-coenzyme A thioesterase 8 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACOT8 |
| 全名 | acyl-CoA thioesterase 8 |
| 背景介绍 | The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010] |
| 生物功能 | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (By similarity). May be involved in the metabolic regulation of peroxisome proliferation. [UniProt] |
| 预测分子量 | 36 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57045 anti-ACOT8 antibody [4D10] FACS image
Flow Cytometry: CTLL-2 cell line stained with ARG57045 anti-ACOT8 antibody [4D10] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57045 anti-ACOT8 antibody [4D10] WB image
Western blot: 1) 10 ng of Recombinant Human ACOT8, 40 µg of 2) Ramos cell lysate, 3) A431 cell lysate, 4) 293T cell lysate, 5) A549 cell lysate stained with ARG57045 anti-ACOT8 antibody [4D10] at 1:1000.
