ARG57015
anti-ACOT7 antibody [1D5]
anti-ACOT7 antibody [1D5] for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1D5] recognizes ACOT7 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1D5 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | ACOT7 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-370 of Human ACOT7. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.1.2.2; CTE-IIa; hBACH; LACH; Cytosolic acyl coenzyme A thioester hydrolase; CTE-II; ACH1; BACH; Long chain acyl-CoA thioester hydrolase; LACH1; Acyl-CoA thioesterase 7; ACT; Brain acyl-CoA hydrolase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O00154 Human Cytosolic acyl coenzyme A thioester hydrolase |
|---|---|
| 基因名称 | ACOT7 |
| 全名 | acyl-CoA thioesterase 7 |
| 背景介绍 | This gene encodes a member of the acyl coenzyme family. The encoded protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this gene may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May play an important physiological function in brain. May play a regulatory role by modulating the cellular levels of fatty acyl-CoA ligands for certain transcription factors as well as the substrates for fatty acid metabolizing enzymes, contributing to lipid homeostasis. Has broad specificity, active towards fatty acyl-CoAs with chain-lengths of C8-C18. Has a maximal activity toward palmitoyl-CoA. [UniProt] |
| 预测分子量 | 42 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG57015 anti-ACOT7 antibody [1D5] ICC/IF image
Immunoflorescense: U87MG cell line stained with ARG57015 anti-ACOT7 antibody [1D5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57015 anti-ACOT7 antibody [1D5] FACS image
Flow Cytometry: 293T cell line stained with ARG57015 anti-ACOT7 antibody [1D5] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57015 anti-ACOT7 antibody [1D5] WB image
Western blot: 30 µg of 293T cell lysate stained with ARG57015 anti-ACOT7 antibody [1D5] at 1:1000.
