ARG42429
anti-ACE antibody [5-369]
anti-ACE antibody [5-369] for Flow cytometry and Human
概述
| 产品描述 | Mouse Monoclonal antibody [5-369] recognizes ACE |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The mouse monoclonal antibody 5-369 recognizes an extracellular epitope of CD143, a 171 kDa type I transmembrane glycoprotein with metallopeptidase activity, expressed mainly on endothelial cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 5-369 |
| 同位型 | IgG1 |
| 靶点名称 | ACE |
| 抗原物种 | Human |
| 抗原 | Dendritic cells. |
| 偶联标记 | Un-conjugated |
| 別名 | DCP1; ICH; ACE; EC 3.2.1.-; MVCD3; Angiotensin-converting enzyme; Dipeptidyl carboxypeptidase I; CD143; CD antigen CD143; EC 3.4.15.1; Kininase II; ACE1; DCP |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 15 mM Sodium azide. |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACE |
| 全名 | angiotensin I converting enzyme |
| 背景介绍 | This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010] |
| 生物功能 | Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. [UniProt] |
| 细胞定位 | Angiotensin-converting enzyme, soluble form: Secreted. Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Detected in both cell membrane and cytoplasm in neurons. [UniProt] |
| 预测分子量 | 150 kDa |
| 翻译后修饰 | Phosphorylated by CK2 on Ser-1299; which allows membrane retention. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG42429 anti-ACE antibody [5-369] FACS image
Flow Cytometry: Human GM-CSF + IL-4 stimulated peripheral blood mononuclear cells stained with ARG42429 anti-ACE antibody [5-369] at 0.6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG42429 anti-ACE antibody [5-369] FACS image
Flow Cytometry: Separation of Human stimulated monocytes (red-filled) from lymphocytes (black-dashed). Human GM-CSF + IL-4 stimulated peripheral blood mononuclear cells stained with ARG42429 anti-ACE antibody [5-369] at 0.6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
