ARG57040
anti-ACAT2 antibody [55D5]
anti-ACAT2 antibody [55D5] for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [55D5] recognizes ACAT2 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 55D5 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | ACAT2 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-397 of Human ACAT2. |
| 偶联标记 | Un-conjugated |
| 別名 | Acetyl-CoA transferase-like protein; EC 2.3.1.9; Acetyl-CoA acetyltransferase, cytosolic; Cytosolic acetoacetyl-CoA thiolase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9BWD1 Human Acetyl-CoA acetyltransferase, cytosolic |
|---|---|
| 基因名称 | ACAT2 |
| 全名 | acetyl-CoA acetyltransferase 2 |
| 背景介绍 | The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014] |
| 预测分子量 | 41 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG57040 anti-ACAT2 antibody [55D5] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG57040 anti-ACAT2 antibody [55D5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57040 anti-ACAT2 antibody [55D5] FACS image
Flow Cytometry: PC3 cell line stained with ARG57040 anti-ACAT2 antibody [55D5] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat antiMouse IgG Alexa fluor 488 conjugate.
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ARG57040 anti-ACAT2 antibody [55D5] WB image
Western blot: 40 µg of 1) HepG2 cell lysate, 2) Ramos cell lysate, 3) MCF-7 cell lysate, 4) HL-60 cell lysate, 5) SW480 cell lysate stained with ARG57040 anti-ACAT2 antibody [55D5] at 1:1000.
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ARG57040 anti-ACAT2 antibody [55D5] WB image
Western blot: 50 ng of Recombinant Human ACAT2 stained with ARG57040 anti-ACAT2 antibody [55D5] at 1:1000.
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ARG57040 anti-ACAT2 antibody [55D5] WB image
Western blot: 5 µg of 1) 293T cell lysate, 2) ACAT2 Transfected 293T cell lysate stained with ARG57040 anti-ACAT2 antibody [55D5] at 1:1000.
