ARG57118
anti-ACAT1 antibody [15E5]
anti-ACAT1 antibody [15E5] for ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [15E5] recognizes ACAT1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 15E5 |
| 同位型 | IgG1, kappa |
| 靶点名称 | ACAT1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 34-427 of Human ACAT1 |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.3.1.9; Acetoacetyl-CoA thiolase; MAT; T2; ACAT; THIL; Acetyl-CoA acetyltransferase, mitochondrial |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P24752 Human Acetyl-CoA acetyltransferase, mitochondrial |
|---|---|
| 基因名称 | ACAT1 |
| 全名 | acetyl-CoA acetyltransferase 1 |
| 背景介绍 | This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009] |
| 生物功能 | Plays a major role in ketone body metabolism. [UniProt] |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity). |
检测图片 (3) Click the Picture to Zoom In
-
ARG57118 anti-ACAT1 antibody [15E5] ICC/IF image
Immunofluorescence: Hep3B cells line stained with ARG57118 anti-ACAT1 antibody [15E5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
-
ARG57118 anti-ACAT1 antibody [15E5] WB image
Western blot: 40 µg of HepG2 and HeLa cell lysates stained with ARG57118 anti-ACAT1 antibody [15E5] at 1:1000.
-
ARG57118 anti-ACAT1 antibody [15E5] WB image
Western blot: 40 µg of 1) Hep3B, 2) 293T, and 3) A431 cell lysates stained with ARG57118 anti-ACAT1 antibody [15E5] at 1:1000.
