ARG45183

anti-ACADSB antibody

anti-ACADSB antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat,Monkey

概述

产品描述 Rabbit Polyclonal antibody recognizes ACADSB
反应物种 Hu, Ms, Rat, Mk
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 ACADSB
抗原物种 Human
抗原 Synthetic peptide corresponding to C-terminal region of human ACADSB.
偶联标记 Un-conjugated
別名 ACADSB; acyl-CoA dehydrogenase, short/branched chain; SBCAD; ACAD7; Short/Branched Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial; Acyl-Coenzyme A Dehydrogenase, Short/Branched Chain; 2-Methyl Branched Chain Acyl-CoA Dehydrogenase; 2-Methylbutyryl-Coenzyme A Dehydrogenase; 2-MEBCAD; 2-Methylbutyryl-CoA Dehydrogenase; EC 1.3.8.5; EC 1.3.99

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 40-44 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.01% Sodium azide and 4% Trehalose.
抗菌剂 0.01% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 25618 Rat ACADSB

GeneID: 36 Human ACADSB

GeneID: 66885 Mouse ACADSB

基因名称 ACADSB
全名 acyl-CoA dehydrogenase, short/branched chain
背景介绍 Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq, Jul 2008]
生物功能 Has greatest activity toward short branched chain acyl-CoA derivative such as (s)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl-CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent. [UniProt]
细胞定位 Mitochondrion matrix.. [UniProt]
预测分子量 47 kDa
翻译后修饰 Acetylation; Phosphoprotein. [UniProt]

检测图片 (6) Click the Picture to Zoom In

  • ARG45183 anti-ACADSB antibody ICC/IF image

    Immunofluorescence: MCF-7 stained with ARG45183 anti-ACADSB antibody at 5 ug/ml dilution.

  • ARG45183 anti-ACADSB antibody WB image

    Western blot: A431, T47D, and A549 stained with ARG45183 anti-ACADSB antibody at 0.5 μg/ml dilution.

  • ARG45183 anti-ACADSB antibody WB image

    Western blot: Rat liver stained with ARG45183 anti-ACADSB antibody at 0.5 μg/ml dilution.

  • ARG45183 anti-ACADSB antibody WB image

    Western blot: Mouse liver stained with ARG45183 anti-ACADSB antibody at 0.5 μg/ml dilution.

  • ARG45183 anti-ACADSB antibody FACS image

    Flow Cytometry: RAW264.7 stained with ARG45183 anti-ACADSB antibody at 1 µg/10^6 cells dilution.

  • ARG45183 anti-ACADSB antibody WB image

    Western blot: Cos-7 stained with ARG45183 anti-ACADSB antibody at 0.5 μg/ml dilution.