ARG43149
anti-ACADS antibody
anti-ACADS antibody for Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ACADS |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ACADS |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-260 of Human ACADS (NP_000008.1). |
| 偶联标记 | Un-conjugated |
| 別名 | EC 1.3.8.1; Butyryl-CoA dehydrogenase; ACAD3; SCAD; Short-chain specific acyl-CoA dehydrogenase, mitochondrial |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HepG2 | ||||||
| 实际分子量 | ~ 42 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACADS |
| 全名 | acyl-CoA dehydrogenase, C-2 to C-3 short chain |
| 背景介绍 | This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014] |
| 生物功能 | Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains (PubMed:21237683, PubMed:11134486). [UniProt] |
| 细胞定位 | Mitochondrion matrix. [UniProt] |
| 预测分子量 | 44 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG43149 anti-ACADS antibody WB image
Western blot: 25 µg of HepG2 cell lysate stained with ARG43149 anti-ACADS antibody at 1:1000 dilution.
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ARG43149 anti-ACADS antibody IP image
Immunoprecipitation: 200 µg extracts of MCF7 cells were immunoprecipitated and stained with ARG43149 anti-ACADS antibody at 1:1000 dilution.
