ARG62665
anti-ABRA1 / CCDC98 antibody [ABRA1-01]
anti-ABRA1 / CCDC98 antibody [ABRA1-01] for Flow cytometry,Immunoprecipitation,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody [ABRA1-01] recognizes ABRA1 / CCDC98 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IP, WB |
| 特异性 | The clone ABRA1-01 recognizes N-terminal part of ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | ABRA1-01 |
| 同位型 | IgG |
| 靶点名称 | ABRA1 / CCDC98 |
| 抗原 | Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag |
| 偶联标记 | Un-conjugated |
| 別名 | Protein FAM175A; Coiled-coil domain-containing protein 98; ABRA1; CCDC98; BRCA1-A complex subunit Abraxas |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Under reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FAM175A |
| 全名 | family with sequence similarity 175, member A |
| 背景介绍 | ABRA1 (Abraxas), also known as CCDC98, is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation. |
| 生物功能 | Involved in DNA damage response and double-strand break (DSB) repair. Component of the BRCA1-A complex, acting as a central scaffold protein that assembles the various components of the complex and mediates the recruitment of BRCA1. The BRCA1-A complex specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesion sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at DSBs. This complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | Phosphorylation of Ser-406 of the pSXXF motif by ATM or ATR constitutes a specific recognition motif for the BRCT domain of BRCA1 (PubMed:17643121, PubMed:17525340, PubMed:17643122). Ionizing radiation promotes rapid phosphorylation at Ser-404 and Ser-406 by ATM; this promotes recruitment of BRCA1 to sites of DNA damage (PubMed:26778126). |
检测图片 (1) Click the Picture to Zoom In
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ARG62665 anti-ABRA1 / CCDC98 antibody [ABRA1-01] WB image
Western blot: ABRA1-EGFP fusion protein transfected HEK293 cell lysate stained with ARG62665 anti-ABRA1 / CCDC98 antibody [ABRA1-01].
