ARG64411
anti-ABCB5 antibody
anti-ABCB5 antibody for Flow cytometry,Western blot and Human
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes ABCB5 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1) |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ABCB5 |
| 抗原物种 | Human |
| 抗原 | C-QTQHRNTSKKAQ |
| 偶联标记 | Un-conjugated |
| 別名 | ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5; ABCB5beta; EST422562; ABCB5alpha |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q2M3G0 Human ATP-binding cassette sub-family B member 5 |
|---|---|
| 背景介绍 | ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008] |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 139 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG64411 anti-ABCB5 antibody WB image
Western Blot: A431 cell lysate (35 µg protein in RIPA buffer). stained with ARG64411 anti-ABCB5 antibody at 0.3 µg/ml dilution.
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ARG64411 anti-ABCB5 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.5% Triton. Cells were stained with ARG64411 anti-ABCB5 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
