ARG52244
anti-AATF phospho (Ser477) antibody
anti-AATF phospho (Ser477) antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes AATF phospho (Ser477) |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Bov, Dog, NHuPrm, Sheep, Xenopus laevis |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AATF |
| 抗原物种 | Human |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | BFR2; Apoptosis-antagonizing transcription factor; CHE1; CHE-1; Rb-binding protein Che-1; Protein AATF; DED |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~66k Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to geneRate the antibody but not by the corresponding dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AATF |
| 全名 | apoptosis antagonizing transcription factor |
| 背景介绍 | Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006). |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody |
| 预测分子量 | 63 kDa |
| 翻译后修饰 | Hyperphosphorylated during the G1/S phase transition. |
检测图片 (1) Click the Picture to Zoom In
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ARG52244 anti-AATF phospho (Ser477) antibody WB image
Western blot: HeLa lysate showing specific immunolabeling of the ~66k Che-1 protein phosphorylated at Ser 477 stained with ARG52244 anti-AATF phospho (Ser477) antibody.
The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen.
