ARG45593

anti-A RAF antibody

anti-A RAF antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes A RAF
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 A RAF
抗原物种 Human
抗原 Recombinant protein containing to human A RAF.
偶联标记 Un-conjugated
別名 ARAF; A-Raf Proto-Oncogene, Serine/Threonine Kinase; A-Raf; ARAF1; V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 1; Serine/Threonine-Protein Kinase A-Raf; Proto-Oncogene A-Raf-1; Proto-Oncogene Pks; EC 2.7.11.1; PKS2; V-Raf Murine Sarcoma 3611 Viral Oncogene-Like Protein; A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase; Ras-Binding Protein DA-Raf; Proto-Oncogene A-Raf; Oncogene ARAF1; EC 2.7.11; RAFA1; PKS

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.1-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 68 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂 0.05% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11836 Mouse ARAF

GeneID: 369 Human ARAF

GeneID: 64363 Rat ARAF

基因名称 ARAF
全名 A-Raf Proto-Oncogene, Serine/Threonine Kinase
背景介绍 Enables protein serine/threonine kinase activity. Involved in negative regulation of apoptotic process; regulation of TOR signaling; and regulation of protein metabolic process. Predicted to be active in cytosol and mitochondrion. Biomarker of high grade glioma. [provided by Alliance of Genome Resources, Feb 2025]
生物功能 Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2. [UniProt]
细胞定位 Cytosol; Mitochondrion. [UniProt]
预测分子量 68 kDa
翻译后修饰 Phosphoprotein. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG45593 anti-A RAF antibody ICC/IF image

    Immunofluorescence: MCF-7 stained with ARG45593 anti-A RAF antibody at 5 μg/ml dilution.

  • ARG45593 anti-A RAF antibody WB image

    Western blot: Hela and MCF-7 stained with ARG45593 anti-A RAF antibody at 0.5 μg/ml dilution.

  • ARG45593 anti-A RAF antibody FACS image

    Flow Cytometry: SiHa stained with ARG45593 anti-A RAF antibody at 1 µg/10^6 cells dilution.

  • ARG45593 anti-A RAF antibody WB image

    Western blot: Rat testis stained with ARG45593 anti-A RAF antibody at 0.5 μg/ml dilution.

  • ARG45593 anti-A RAF antibody WB image

    Western blot: Mouse testis stained with ARG45593 anti-A RAF antibody at 0.5 μg/ml dilution.