ARG57235
anti-5-methylcytosine / 5-mC antibody [RM231]
anti-5-methylcytosine / 5-mC antibody [RM231] for Dot blot,ELISA,ICC/IF,MeDIP and Other
概述
| 产品描述 | Rabbit Monoclonal antibody [RM231] recognizes 5-methylcytosine / 5-mC |
|---|---|
| 反应物种 | Other |
| 应用 | Dot, ELISA, ICC/IF, MeDIP |
| 特异性 | This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA. |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | RM231 |
| 同位型 | IgG |
| 靶点名称 | 5-methylcytosine / 5-mC |
| 抗原物种 | Others |
| 抗原 | BSA-conjugated 5-methylcytosine. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.09% Sodium azide, 50% Glycerol and 1% BSA. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol and 1% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (5) Click the Picture to Zoom In
-
ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
-
ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] ELISA image
ELISA: Titration curve of ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231]. Antigen: The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA.
Secondary antibody: An alkaline phosphatase conjugated anti-rabbit IgG.
-
ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] Dot blot image
Dot blot: Double stranded DNA using ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231]. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231].
-
ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] ELISA image
Direct ELISA: The plate was directly coated with different concentrations of genomic DNA isolated from HeLa cells. 1 µg/ml or 3 µg/ml of ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] was used as the primary antibody, and a HRR-conjugated anti-rabbit IgG as the secondary antibody.
-
ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] MeDIP image
MeDIP: ARG57235 anti-5-methylcytosine / 5-mC antibody [RM231] at a 2:1 DNA:Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1 µg of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.
