ARG23717

anti-4 Hydroxynonenal antibody [12F7]

anti-4 Hydroxynonenal antibody [12F7] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Other

Ferroptosis/Oxytosis Study antibody; Lipid peroxidation Marker antibody
publication_link 参考文献8

概述

产品描述 Mouse Monoclonal antibody [12F7] recognizes 4 Hydroxynonenal
反应物种 Other
应用 ELISA, ICC/IF, IHC-P, WB
特异性 Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
宿主 Mouse
克隆 Monoclonal
克隆号 12F7
同位型 IgG1
靶点名称 4 Hydroxynonenal
抗原物种 Others
抗原 Synthetic 4-Hydroxynonenal modified KLH.
偶联标记 Un-conjugated
別名 4-HNE

应用说明

应用建议
应用 推荐稀释比
ELISA1:1000
ICC/IF1:50
IHC-P1:50
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol.
抗菌剂 0.09% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

产品亮点 Related Antibody Duos and Panels:
ARG30337 Ferroptosis / Oxytosis Antibody Panel
Related products:
Hydroxynonenal antibodies; Hydroxynonenal ELISA Kits; Hydroxynonenal Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Ferroptosis/Oxytosis Antibody Panel is launched
Pericytes, new therapeutic target for Alzheimer's disease?
Therapeutic strategies against PDAC
研究领域 Ferroptosis/Oxytosis Study antibody; Lipid peroxidation Marker antibody

检测图片 (3) Click the Picture to Zoom In

  • ARG23717 anti-4 Hydroxynonenal antibody [12F7] IHC-P image

    Immunohistochemistry: Mouse testis stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:50 dilution.

    From Lipeng Li et al. FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice (2023), doi: 10.1016/j.ecoenv.2023.115309, Fig. 4A1.

  • ARG23717 anti-4 Hydroxynonenal antibody [12F7] ICC/IF image

    Immunofluorescence: HEK293 cells fixed with 5% Formaldehyde (5 min). Cells were stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:50 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G): Untreated. (B,D,F,H): Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Primary antibodies. (G,H) Composite.

  • ARG23717 anti-4 Hydroxynonenal antibody [12F7] WB image

    Western blot: 0.5 µg of BSA, 0.5 µg of 4-hydroxyl nonenal-BSA, 2.0 µg of 4-hydroxy nonenal-BSA, 0.5 µg of 4-hydroxy-2-hexenal and 2.0 µg of 4-hydroxy-2-hexenal stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:1000 for 2 hours at RT.

文献引用

Mechanism of YY1 mediating autophagy dependent ferroptosis in PM2.5 induced cardiac fibrosis

IHC-P / Mouse

Huaifang Hu et al.
Chemosphere.,  (2023)

publication_link

 

hr_line

Hydrogen Attenuates Chronic Intermittent Hypoxia-Induced Cardiac Hypertrophy by Regulating Iron Metabolism

WB / Mouse

Jixian Song et al.
Curr Issues Mol Biol.,  (2023)

publication_link

 

hr_line

FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice

IHC-P / Mouse

Lipeng Li et al.
Ecotoxicol Environ Saf.,  (2023)

publication_link

 

hr_line

A novel quinoline derivative, DFIQ, sensitizes NSCLC cells to ferroptosis by promoting oxidative stress accompanied by autophagic dysfunction and mitochondrial damage

WB / Human

Yung-Ding Bow et al.
Cancer Cell Int.,  (2023)

publication_link

 

hr_line

Effects of liraglutide on astrocyte polarization and neuroinflammation in db/db mice: focus on iron overload and oxidative stress

IHC-P / Mouse

Ji-Ren An et al.
Front Cell Neurosci.,  (2023)

publication_link

 

hr_line

Liraglutide attenuates hepatic iron levels and ferroptosis in db/db mice

WB / Mouse

Ji-Xian Song et al.
Bioengineered.,  (2022)

publication_link

 

hr_line

Fermented and Aged Ginseng Sprouts ( Panax ginseng) and Their Main Component, Compound K, Alleviate Asthma Parameters in a Mouse Model of Allergic Asthma through Suppression of Inflammation, Apoptosis, ER Stress, and Ferroptosis

WB / Mouse

Ji Hyeon Ryu et al.
Antioxidants (Basel),  (2022)

publication_link

 

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Oyster-Derived Tyr-Ala (YA) Peptide Prevents Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure by Suppressing Inflammatory, Apoptotic, Ferroptotic, and Pyroptotic Signals

WB / Mouse

Adrian S Siregar et al.
Mar Drugs.,  (2021)

publication_link

 

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