anti-4 Hydroxynonenal antibody [12F7]
anti-4 Hydroxynonenal antibody [12F7] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Other
Ferroptosis/Oxytosis Study antibody; Lipid peroxidation Marker antibody
|产品描述||Mouse Monoclonal antibody [12F7] recognizes 4 Hydroxynonenal|
|应用||ELISA, ICC/IF, IHC-P, WB|
|特异性||Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.|
|抗原||Synthetic 4-Hydroxynonenal modified KLH.|
|应用说明||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|纯化||Purification with Protein G.|
|缓冲液||PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol.|
|抗菌剂||0.09% Sodium azide|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|产品亮点||Related Antibody Duos and Panels:
ARG30337 Ferroptosis / Oxytosis Antibody Panel
Hydroxynonenal antibodies; Hydroxynonenal ELISA Kits; Hydroxynonenal Duos / Panels; Anti-Mouse IgG secondary antibodies;
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|研究领域||Ferroptosis/Oxytosis Study antibody; Lipid peroxidation Marker antibody|
检测图片 (3) Click the Picture to Zoom In
ARG23717 anti-4 Hydroxynonenal antibody [12F7] IHC-P image
Immunohistochemistry: Mouse testis stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:50 dilution.
From Lipeng Li et al. FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice (2023), doi: 10.1016/j.ecoenv.2023.115309, Fig. 4A1.
ARG23717 anti-4 Hydroxynonenal antibody [12F7] ICC/IF image
Immunofluorescence: HEK293 cells fixed with 5% Formaldehyde (5 min). Cells were stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:50 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G): Untreated. (B,D,F,H): Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Primary antibodies. (G,H) Composite.
ARG23717 anti-4 Hydroxynonenal antibody [12F7] WB image
Western blot: 0.5 µg of BSA, 0.5 µg of 4-hydroxyl nonenal-BSA, 2.0 µg of 4-hydroxy nonenal-BSA, 0.5 µg of 4-hydroxy-2-hexenal and 2.0 µg of 4-hydroxy-2-hexenal stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:1000 for 2 hours at RT.