ARG63881
anti-14-3-3 sigma / Stratifin antibody
anti-14-3-3 sigma / Stratifin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes 14-3-3 sigma / Stratifin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Dog, Pig |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | 14-3-3 sigma / Stratifin |
| 抗原物种 | Human |
| 抗原 | C-DLHTLSEDSYKDST |
| 偶联标记 | Un-conjugated |
| 別名 | YWHAS; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SFN |
| 全名 | stratifin |
| 生物功能 | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. p53-regulated inhibitor of G2/M progression. [UniProt] |
| 产品亮点 | Related products: 1433 sigma antibodies; 1433 sigma Duos / Panels; Anti-Goat IgG secondary antibodies; Related news: 14-3-3η as a promising target for the treatment of Major Depression Disorder Has “Obesity gene” been found? |
| 研究领域 | Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. |
检测图片 (3) Click the Picture to Zoom In
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ARG63881 anti-14-3-3 sigma / Stratifin antibody WB image
Western blot: 30 µg of Mouse lung lysate stained with ARG63881 anti-14-3-3 sigma / Stratifin antibody at 1:1500 dilution.
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ARG63881 anti-14-3-3 sigma / Stratifin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skin tissue. Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG63881 anti-14-3-3 sigma / Stratifin antibody at 2 µg/ml dilution followed by HRP-staining.
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ARG63881 anti-14-3-3 sigma / Stratifin antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Skin. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63881 anti-14-3-3 sigma / Stratifin antibody at 2.5 µg/ml dilution followed by AP-staining.
客户反馈
Good
anti-14-3-3 sigma / Stratifin antibody
Application：WB
Sample：Rat lung
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-14-3-3 sigma / Stratifin antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
