ARG54732
anti-14-3-3 gamma antibody
anti-14-3-3 gamma antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes 14-3-3 gamma |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Bov, Rat, Chk, Xenopus |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | 14-3-3 gamma |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 136-164 (Center) of Human 14-3-3 gamma (NP_036611.2). |
| 偶联标记 | Un-conjugated |
| 別名 | 14-3-3 protein gamma; PPP1R170; 14-3-3GAMMA; KCIP-1; Protein kinase C inhibitor protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse cerebellum |
属性
| 纯化 | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
|---|---|
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | YWHAG |
| 全名 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma |
| 背景介绍 | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008] |
| 生物功能 | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. [From Uniprot] |
| 细胞定位 | Cytoplasm. |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | Phosphorylated by various PKC isozymes. |
检测图片 (3) Click the Picture to Zoom In
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ARG54732 anti-14-3-3 gamma antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon carcinoma stained with ARG54732 anti-14-3-3 gamma antibody.
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ARG54732 anti-14-3-3 gamma antibody WB image
Western blot: 35 µg of Mouse cerebellum lysate stained with ARG54732 anti-14-3-3 gamma antibody.
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ARG54732 anti-14-3-3 gamma antibody FACS image
Flow Cytometry: WiDr cells stained with ARG54732 anti-14-3-3 gamma antibody (bottom histogram) or without primary antibody control (top histogram).
客户反馈
Average
anti-14-3-3 gamma antibody
Application：WB
Sample：HeLa
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
