ARG57090
anti-14-3-3 epsilon antibody [4F8]
anti-14-3-3 epsilon antibody [4F8] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [4F8] recognizes 14-3-3 epsilon |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4F8 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | 14-3-3 epsilon |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-255 of Human 14-3-3 epsilon |
| 偶联标记 | Un-conjugated |
| 別名 | KCIP-1; HEL2; MDCR; 14-3-3 protein epsilon; 14-3-3E; MDS |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | YWHAE |
| 全名 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon |
| 背景介绍 | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008] |
| 生物功能 | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. [UniProt] |
| 预测分子量 | 29 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57090 anti-14-3-3 epsilon antibody [4F8] WB image
Western blot: 40 µg of 1) HeLa, 2) Jurkat, 3) 293T, 4) A549, and 5) Mouse Brain Tissue lysate stained with ARG57090 anti-14-3-3 epsilon antibody [4F8] at 1:1000.
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ARG57090 anti-14-3-3 epsilon antibody [4F8] WB image
Western blot: 50 ng of 1) 14-3-3 Zeta, 2) 14-3-3 Beta, 3) 14-3-3 Epsilon, 4) 14-3-3 Eta, 5) 14-3-3 Gamma, 6) 14-3-3 Sigma, and 7) 14-3-3 Tau recombinant proteins stained with ARG57090 anti-14-3-3 epsilon antibody [4F8] at 1:1000.
