ARG57827
anti-14-3-3 antibody
anti-14-3-3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes 14-3-3 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | 14-3-3 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide within aa. 217-246 of Human 14-3-3. |
| 偶联标记 | Un-conjugated |
| 別名 | Protein 1054; 14-3-3 protein beta/alpha; Protein kinase C inhibitor protein 1; HS1; GW128; KCIP-1; YWHAA; HEL-S-1 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Jurkat | ||||||||
| 实际分子量 | ~ 28 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Saturated Ammonium Sulfate precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | YWHAB |
| 全名 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta |
| 背景介绍 | This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. [UniProt] |
| 细胞定位 | Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt] |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
-
ARG57827 anti-14-3-3 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG57827 anti-14-3-3 antibody (green). DAPI (blue) for nuclear staining.
-
ARG57827 anti-14-3-3 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma tissue stained with ARG57827 anti-14-3-3 antibody.
-
ARG57827 anti-14-3-3 antibody WB image
Western blot: Jurkat cell lysate stained with ARG57827 anti-14-3-3 antibody.
