ARG52214
anti-14-3-3 antibody
anti-14-3-3 antibody for Western blot and Rat
Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes 14-3-3 |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Bov, Chk, Dog, NHuPrm, Xenopus laevis, Zfsh |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | 14-3-3 |
| 抗原物种 | Rat |
| 抗原 | Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | Protein 1054; 14-3-3 protein beta/alpha; Protein kinase C inhibitor protein 1; HS1; GW128; KCIP-1; YWHAA; HEL-S-1 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~29k 14-3-3 protein. Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption of the antibody with the peptide that was used to geneRate the antibody. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | YWHAB |
| 全名 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta |
| 背景介绍 | 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. |
| 研究领域 | Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. |
检测图片 (1) Click the Picture to Zoom In
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ARG52214 anti-14-3-3 antibody WB image
Western Blot: rat hippocampal lysate showing specific immunolabeling of the ~29k 14-3-3 protein stained with 14-3-3 antibody (ARG52214).
