ARG41003
anti-14-3-3 alpha + beta antibody
anti-14-3-3 alpha + beta antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes 14-3-3 alpha + beta |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 特异性 | This antibody might also react to other 14-3-3 proteins based on sequence alalysis: 14-3-3 protein zeta/delta (88%), 14-3-3 protein theta (82%), 14-3-3 protein gamma (75%), 14-3-3 protein eta (76%). |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | 14-3-3 alpha + beta |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-246 of Human 14-3-3 beta (NP_647539.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Protein 1054; 14-3-3 protein beta/alpha; Protein kinase C inhibitor protein 1; HS1; GW128; KCIP-1; YWHAA; HEL-S-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 28 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | YWHAB |
| 全名 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta |
| 背景介绍 | This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. [UniProt] |
| 细胞定位 | Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt] |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG41003 anti-14-3-3 alpha + beta antibody IHC-P image
Immunohistochemistry: Human tonsil stained with ARG41003 anti-14-3-3 alpha + beta antibody at 1:200 dilution.
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ARG41003 anti-14-3-3 alpha + beta antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG41003 anti-14-3-3 alpha + beta antibody at 1:100 dilution.
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ARG41003 anti-14-3-3 alpha + beta antibody WB image
Western blot: Mouse Liver and Mouse Kidney stained with ARG41003 anti-14-3-3 alpha + beta antibody at 1:1000 dilution.
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ARG41003 anti-14-3-3 alpha + beta antibody WB image
Western blot: HepG2 stained with ARG41003 anti-14-3-3 alpha + beta antibody at 1:1000 dilution.
