ARG30334

Tumor-infiltrating Lymphocyte Antibody Panel

Tumor infiltrating Lymphocyte markers; Immunology; anti-tumor immune responses; tumor immune infiltration

内含物

货号 内含物名称 宿主克隆性 反应 应用 包装
ARG65859 anti-CD3 antibody [SQab1713] Rabbit mAb Hu FACS, ICC/IF, IHC-P, IP, WB 20 μl
ARG66628 anti-CD8 antibody [SQab19146] Rabbit mAb Hu IHC-P 20 μl
ARG65860 anti-CD4 antibody [SQab1714] Rabbit mAb Hu FACS, IHC-P, IP, WB 20 μl
ARG66197 anti-CD20 antibody [SQab1719] Rabbit mAb Hu FACS, ICC/IF, IHC-P, IP 20 μl

概述

产品描述 Tumor-infiltrating Lymphocyte (TIL) Antibody Panel is an all-in-one solution to make identification of TILs easy and economic. This antibody panel comprises antibodies against CD3, CD4, CD8 and CD20 for identifying general T cells, Treg cells, cytotoxic T cells and B cells, respectively. All the antibodies in this panel have excellent IHC staining performance.

Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
靶点名称 Tumor-infiltrating Lymphocyte
全名 Antibody Panel for Tumor-infiltrating Lymphocyte
別名 Tumor-infiltrating Lymphocyte antibody; CD3 antibody; CD4 antibody; CD20 antibody; CD8 antibody

属性

存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

研究领域 Tumor infiltrating Lymphocyte markers; Immunology; anti-tumor immune responses; tumor immune infiltration

检测图片 (17) Click the Picture to Zoom In

  • ARG65859 anti-CD3 antibody [SQab1713] WB image

    Western blot: 30 µg of Molt4 cell lysate stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:500 dilution.

  • ARG65860 anti-CD4 antibody [SQab1714] WB image

    Western blot: 30 µg of Jurkat and Molt4 cell lysates stained with ARG65860 anti-CD4 antibody [SQab1714] at 1:500 dilution.

  • ARG65859 anti-CD3 antibody [SQab1713] ICC/IF image

    Immunofluorescence: Jurkat cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG65859 anti-CD3 antibody [SQab1713] (green) at 1:50 and 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.

  • ARG66197 anti-CD20 antibody [SQab1719] ICC/IF image

    Immunofluorescence: Raji cells fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG66197 anti-CD20 antibody [SQab1719] (green) at 1:1000 at 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.

  • ARG65859 anti-CD3 antibody [SQab1713] FACS image

    Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG65859 anti-CD3 antibody [SQab1713] (blue) at 1:1000 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.

  • ARG65860 anti-CD4 antibody [SQab1714] FACS image

    Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG65860 anti-CD4 antibody [SQab1714] (blue) at 1:50 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.

  • ARG66197 anti-CD20 antibody [SQab1719] FACS image

    Flow Cytometry: Raji cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG66197 anti-CD20 antibody [SQab1719] (red) at 1:500 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG65859 anti-CD3 antibody [SQab1713] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil tissue stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG65860 anti-CD4 antibody [SQab1714] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil tissue stained with ARG65860 anti-CD4 antibody [SQab1714] at 1:2000 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG66197 anti-CD20 antibody [SQab1719] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human appendix tissue stained with ARG66197 anti-CD20 antibody [SQab1719] at 1:20000 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66628 anti-CD8 antibody [SQab19146] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human tonsil tissue stained with ARG66628 anti-CD8 antibody [SQab19146]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).

  • ARG65859 anti-CD3 antibody [SQab1713] IP image

    Immunoprecipitation: 0.4 mg of Molt-4 whole cell lysate was immunoprecipitated (1:15 dilution) and stained with ARG65859 anti-CD3 antibody [SQab1713].

    Lane 1: Immunoprecipitation in Molt-4 whole cell lysate
    Lane 2: Rabbit IgG instead of Primary Ab in Molt-4 whole cell lysate
    Lane 3: Molt-4 whole cell lysate, 10 µg (input)

  • ARG65860 anti-CD4 antibody [SQab1714] IP image

    Immunoprecipitation: 0.4 mg of Molt-4 whole cell lysate was immunoprecipitated (1:50 dilution) and stained with ARG65860 anti-CD4 antibody [SQab1714].

    Lane 1: Immunoprecipitation in Molt-4 whole cell lysate
    Lane 2: Rabbit IgG instead of Primary Ab in Molt-4 whole cell lysate
    Lane 3: Molt-4 whole cell lysate, 10 µg (input)

  • ARG66197 anti-CD20 antibody [SQab1719] IP image

    Immunoprecipitation: 0.4 mg of Raji whole cell lysate was immunoprecipitated (1:20 dilution) and stained with ARG66197 anti-CD20 antibody [SQab1719].

    Lane 1: Immunoprecipitation in Raji whole cell lysate
    Lane 2: PBS instead of Primary Ab in Raji whole cell lysate
    Lane 3: Raji whole cell lysate, 10 µg (input)

  • ARG65859 anti-CD3 antibody [SQab1713] WB image

    Western blot: 20 µg of Jurkat and Mouse spleen (untreated or treated with LPS) lysates stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:1000 dilution, overnight at 4°C.

  • ARG65859 anti-CD3 antibody [SQab1713] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human colon tissue stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG66197 anti-CD20 antibody [SQab1719] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human spleen tissue stained with ARG66197 anti-CD20 antibody [SQab1719] at 1:20000 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0