ARG81353
SOD Activity Assay Kit
SOD Activity Assay 试剂盒 for Functional study and Human,Mouse,Rat,Mammal
参考文献1
概述
| 产品描述 | ARG81353 SOD Activity Assay Kit is a detection kit for the quantification of Superoxide Dismutase in serum, plasma and cell / tissue lysates. |
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| 反应物种 | Hu, Ms, Rat, Mamm |
| 应用 | FuncSt |
| 靶点名称 | SOD |
| 偶联标记说明 | Read at 490 nm. |
| 样品类型 | Serum, plasma and cell / tissue lysates. |
| 标准范围 | 0.0012 - 5 U/µl (Unit Definition: One unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and xanthine oxidase, at pH 7.8 at 25°C in a 3.0 ml reaction volume.) |
| 样本量 | 10 - 70 µl |
| 別名 | homodimer; EC 1.15.1.1; SOD; HEL-S-44; Superoxide dismutase [Cu-Zn]; ALS1; Superoxide dismutase 1; IPOA; ALS; hSod1 |
应用说明
| 应用说明 | Please note that this kit does not include a microplate. |
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| 检测时间 | 1 hour |
属性
| 形式 | Liquid |
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| 存放说明 | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SOD1 |
| 全名 | superoxide dismutase 1, soluble |
| 背景介绍 | The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Destroys radicals which are normally produced within the cells and which are toxic to biological systems. [UniProt] |
| 翻译后修饰 | Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation. The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. Palmitoylation helps nuclear targeting and decreases catalytic activity. Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81353 SOD Activity Assay Kit standard curve image
ARG81353 SOD Activity Assay Kit results of a typical standard run with optical density reading at 490 nm.
文献引用
Isoliquiritigenin Prevents the Development of Nephropathy by an HFD in Rats Through the Induction of Antioxidant Production and Inhibition of the MD-2/TLR4/NF-κB Pathway
Functional assay / Rat
| Title | Download Link |
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| ARG81353 SOD Activity Assay Kit User's manual |
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