ARG70344
SARS-CoV-2 Spike recombinant protein (S1 Subunit) (Fc-His-tagged, C-ter)
SARS-CoV-2 Spike recombinant protein (S1 Subunit) (Fc-His-tagged, C-ter) for Binding Activity,SDS-PAGE and Virus
概述
| 产品描述 | HEK293 expressed, Fc-His-tagged (C-ter) SARS-CoV-2 Spike recombinant protein (S1 Subunit). |
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| 反应物种 | Virus |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | SARS-CoV-2 Spike protein (S1 Subunit) |
| 物种 | Virus |
| A.A. 序列 | Val11 - Arg682 of SARS - CoV - 2 Spike protein (S1 Subunit) (YP_009724390.1) with an Fc - 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2µg/ml (100 µl/well) can bind Recombinant SARS-CoV-2 Spike S1, the EC50 of SARS-COV-2 Spike S1 is 6.79 ng/ml. Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 90.8 nM as determined in a SPR assay. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 90% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (1) Click the Picture to Zoom In
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ARG70344 SARS-CoV-2 Spike recombinant protein (S1 Subunit) (Fc-His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70344 SARS-CoV-2 Spike recombinant protein (S1 Subunit) (Fc-His-tagged, C-ter).
