Rat TNF alpha ELISA Kit

Rat TNF alpha ELISA 试剂盒 for ELISA and Rat

Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit; Signaling Transduction kit
publication_link 参考文献1


货号 内含物名称 包装 温度
ARG80241-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80241-002 Standard (Lyophilized) 3 X 3.2 ng/vial 4°C
ARG80241-003 Standard diluent buffer 20 ml 4°C
ARG80241-004 Antibody conjugate concentrate 400 µl 4°C
ARG80241-005 Antibody diluent buffer 16 ml 4°C
ARG80241-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80241-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80241-008 20X Wash buffer 50 ml 4°C
ARG80241-009 TMB substrate 12 ml 4°C (Protect from light)
ARG80241-010 STOP solution 12 ml 4°C
ARG80241-011 Plate sealer 4 strips Room temperature



ARG80241 Rat TNF alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat TNF alpha in Serum, Plasma, Cell culture supernatants.

反应物种 Rat
特异性 No significant cross-reactivity or interference with Human TNF- alpha,TNF- beta;porcine TNF- alpha
靶点名称 TNF alpha
偶联标记 HRP

Substrate: TMB and read at 450 nm

灵敏度 30 pg/ml
样品类型 Serum, Plasma, Cell culture supernatants
标准范围 62.5 - 4000 pg/ml
样本量 100 µl

CV: <10%

別名 Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF


检测时间 4 hours


形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 24835 Rat TNF

Swiss-port # P16599 Rat Tumor necrosis factor

基因名称 LOC103694380
全名 tumor necrosis factor
背景介绍 Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
产品亮点 Related products:
TNF alpha antibodies; TNF alpha ELISA Kits; TNF alpha Duos / Panels; TNF alpha recombinant proteins;
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研究领域 Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit; Signaling Transduction kit
翻译后修饰 The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

检测图片 (1) Click the Picture to Zoom In

  • ARG80241 Rat TNF alpha ELISA Kit standard curve image

    ARG80241 Rat TNF alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.


Dexmedetomidine protects against burn-induced intestinal barrier injury via the MLCK/p-MLC signalling pathway


Chao Qin et al.
Burns.,  (2021)



Title Download Link
ARG80241 Rat TNF alpha ELISA Kit User's manual download icon Download