ARG82734
Rat Legumain (total) ELISA Kit
Rat Legumain (total) ELISA 试剂盒 for ELISA and Rat
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82734-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82734-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82734-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82734-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82734-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82734-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82734-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82734-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82734-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82734-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82734-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82734 Rat Legumain (total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Legumain (total) in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 靶点名称 | Legumain (total) |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 16 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.1% Inter-Assay CV: 6.6% |
| 別名 | PRSC1; AEP; Protease, cysteine 1; LGMN1; Asparaginyl endopeptidase; Legumain; EC 3.4.22.34 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LGMN |
| 全名 | legumain |
| 背景介绍 | This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008] |
| 生物功能 | Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. [UniProt] |
| 细胞定位 | Lysosome. [UniProt] |
| 产品亮点 | Related products: Legumain antibodies; Legumain ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Glycosylated. Activated by autocatalytic processing at pH 4. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82734 Rat Legumain (total) ELISA Kit standard curve image
ARG82734 Rat Legumain (total) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82734 Rat Legumain (total) ELISA Kit User manual |
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