ARG81648
Rat GDNF ELISA Kit
Rat GDNF ELISA 试剂盒 for ELISA and Rat
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81648-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81648-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81648-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81648-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81648-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81648-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81648-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81648-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81648-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81648-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81648-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81648 Rat GDNF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat GDNF in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | GDNF |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.6%; Inter-Assay CV: 7.7% |
| 別名 | ATF; Astrocyte-derived trophic factor; HSCR3; hGDNF; Glial cell line-derived neurotrophic factor; HFB1-GDNF; ATF1; ATF2 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q07731 Rat Glial cell line-derived neurotrophic factor |
|---|---|
| 基因名称 | GDNF |
| 全名 | glial cell derived neurotrophic factor |
| 背景介绍 | This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010] |
| 生物功能 | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. [UniProt] |
| 产品亮点 | Related products: GDNF antibodies; GDNF ELISA Kits; GDNF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG81648 Rat GDNF ELISA Kit standard curve image
ARG81648 Rat GDNF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81648 Rat GDNF ELISA Kit User's manual |
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