ARG82105
Rat FGF23 ELISA Kit
Rat FGF23 ELISA 试剂盒 for ELISA and Rat
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82105-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82105-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82105-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82105-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82105-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82105-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82105-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82105-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82105-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82105-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82105-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82105 Rat FGF23 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat FGF23 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | FGF23 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.9%; Inter-Assay CV: 6.5% |
| 別名 | ADHR; Phosphatonin; HPDR2; FGFN; Tumor-derived hypophosphatemia-inducing factor; PHPTC; FGF-23; Fibroblast growth factor 23; HYPF |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FGF23 |
| 全名 | fibroblast growth factor 23 |
| 背景介绍 | This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). [provided by RefSeq, Feb 2013] |
| 生物功能 | Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. [UniProt] |
| 细胞定位 | Secreted. Note=Secretion is dependent on O-glycosylation. [UniProt] |
| 产品亮点 | Related products: FGF23 antibodies; FGF23 ELISA Kits; FGF23 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases. O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82105 Rat FGF23 ELISA Kit standard curve image
ARG82105 Rat FGF23 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82105 Rat FGF23 ELISA kit User manual |
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