ARG82348
Rat DKK1 ELISA Kit
Rat DKK1 ELISA 试剂盒 for ELISA and Rat
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82348-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82348-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82348-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82348-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82348-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82348-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82348-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82348-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82348-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82348-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82348-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82348 Rat DKK1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat DKK1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 靶点名称 | DKK1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 23.45 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 46.9 - 3000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 7.5% Inter-Assay CV: 9.0% |
| 別名 | Dkk-1; Dickkopf-1; SK; DKK-1; hDkk-1; Dickkopf-related protein 1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | DKK1 |
|---|---|
| 全名 | dickkopf WNT signaling pathway inhibitor 1 |
| 背景介绍 | This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008] |
| 生物功能 | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: DKK1 antibodies; DKK1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG82348 Rat DKK1 ELISA Kit standard curve image
ARG82348 Rat DKK1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82348 Rat DKK1 ELISA Kit User manual |
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