ARG82079
Rat CNTF ELISA Kit
Rat CNTF ELISA 试剂盒 for ELISA and Rat
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82079-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82079-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82079-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82079-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82079-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82079-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82079-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82079-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82079-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82079-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82079-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82079 Rat CNTF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CNTF in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CNTF |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.4%; Inter-Assay CV: 7.4% |
| 別名 | HCNTF; CNTF; Ciliary neurotrophic factor |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CNTF |
| 全名 | ciliary neurotrophic factor |
| 背景介绍 | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010] |
| 生物功能 | CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related products: CNTF antibodies; CNTF ELISA Kits; CNTF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG82079 Rat CNTF ELISA Kit standard curve image
ARG82079 Rat CNTF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82079 Rat CNTF ELISA Kit User's manual |
 Download
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