ARG82855
Rat CES3 ELISA Kit
Rat CES3 ELISA 试剂盒 for ELISA and Rat
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82855-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82855-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82855-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82855-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82855-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82855-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82855-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82855-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82855-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82855-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82855-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82855 Rat CES3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CES3 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 靶点名称 | CES3 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.8% Inter-Assay CV: 6.4% |
| 別名 | ES31; Liver carboxylesterase 31 homolog; Carboxylesterase 3; EC 3.1.1.1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | CES3 |
|---|---|
| 全名 | carboxylesterase 3 |
| 背景介绍 | This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined. [provided by RefSeq, Jun 2010] |
| 生物功能 | Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows low catalytic efficiency for hydrolysis of CPT-11 (7-ethyl-10-[4-(1-piperidino)-1-piperidino]-carbonyloxycamptothecin), a prodrug for camptothecin used in cancer therapeutics. [UniProt] |
| 细胞定位 | Endoplasmic reticulum lumen. [UniProt] |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82855 Rat CES3 ELISA Kit standard curve image
ARG82855 Rat CES3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82855 Rat CES3 ELISA Kit User manual |
 Download
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