ARG83378

Pyrophosphate Assay Kit

Pyrophosphate Assay 试剂盒 for Functional study and Other

Pyrophosphates, Anions, Dietary minerals, Molecular biology, Nucleotides, E-number additives

概述

产品描述 ARG83378 Pyrophosphate Assay Kit is an Assay kit for the quantification of Pyrophosphate in Serum, Plasma, Saliva, Urine and Cell / Tissue lysates
反应物种 Other
应用 FuncSt
靶点名称 Pyrophosphate
偶联标记 Un-conjugated
偶联标记说明 Read at 590 nm.
灵敏度 2.5 µM
样品类型 Serum, Plasma, Saliva, Urine and Cell / Tissue lysates
标准范围 3.91 - 125 µM
样本量 50 µL

应用说明

检测时间 ~30 min

属性

形式 Liquid
存放说明 Store components at -20°C, -80°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

背景介绍 Pyrophosphate is a potent inhibitor of calcium-phosphate crystal formation and growth. Extracellular pyrophosphate is produced upon ATP hydrolysis by the enzyme ectonucleotide pyrophosphatase/phosphodiesterase 1 (eNPP1). Pyrophosphate is degraded to phosphate by tissue-nonspecific alkaline phosphatase (TNAP), promoting calcification. Another enzyme, ectonucleoside triphosphate diphosphohydrolase 1 (eNTPD1), can hydrolyze ATP and ADP to phosphate, reducing the availability of ATP for pyrophosphate production and likely inducing calcification.
生物功能 The anion P2O7 is abbreviated PPi, standing for inorganic pyrophosphate. It is formed by the hydrolysis of ATP into AMP in cells.
研究领域 Pyrophosphates, Anions, Dietary minerals, Molecular biology, Nucleotides, E-number additives

检测图片 (1) Click the Picture to Zoom In

  • ARG83378 Pyrophosphate Assay Kit standard curve image

    ARG83378 Pyrophosphate Assay Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG83378 Pyrophosphate Assay Kit User manual download icon Download