ARG82287

Porcine FGF basic ELISA Kit

Porcine FGF basic ELISA 试剂盒 for ELISA and Pig

概述

产品描述 ARG82287 Porcine FGF basic ELISA Kit is an Enzyme Immunoassay kit for the quantification of Porcine FGF basic in serum and plasma.
反应物种 Pig
应用 ELISA
特异性 Not react with following recombinant porcine proteins: Adiponectin, ApoAI, BMP1, BMP2, BMP3, BMP4, BMP5, BMP7, CCL2, CCL4, CCL5, CRP, HSP27, HGF, IL1 beta, IL1 RA, IL2, IL4, IL5, sFGF BASICR, IL8, IL10, IL12, IL13, IL15, IL17C, IL21, IL23, IFN gamma, MMP2, MMP9, IL2R, PDGF, serpin E1, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TLR9, TNF alpha, TNF RI, TNF RII, VEGF and VEGF R1.
靶点名称 FGF basic
偶联标记 HRP
偶联标记说明 Read at 450 nm.
灵敏度 7.8 pg/ml
样品类型 Serum and plasma.
标准范围 15.6 - 1000 pg/ml
样本量 100 µl
精确 Intra-Assay CV: 6%
Inter-Assay CV: 9%
別名 FGF-2; Fibroblast growth factor 2; bFGF; FGFB; Heparin-binding growth factor 2; BFGF; HBGF-2; Basic fibroblast growth factor

应用说明

检测时间 ~ 3 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称 FGF2
全名 fibroblast growth factor 2 (basic)
背景介绍 The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
生物功能 Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt]
细胞定位 Secreted. Nucleus. Note=Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. [UniProt]
产品亮点 Related products:
FGF basic antibodies; FGF basic ELISA Kits; FGF basic recombinant proteins;
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The role of HDGF in tumor angiogenesis
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
翻译后修饰 Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.

Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG82287 Porcine FGF basic ELISA Kit standard curve image

    ARG82287 Porcine FGF basic ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82287 Porcine FGF basic ELISA Kit User manual download icon Download