ARG63045
Mouse anti-Human Kappa Light Chain antibody [MEM-09]
Mouse anti-Human Kappa Light Chain 抗体 [MEM-09] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [MEM-09] recognizes Human Kappa Light Chain |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, FACS, ICC/IF, IHC-P, IP, WB |
| 特异性 | The clone MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa). Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-09 |
| 同位型 | IgG1 |
| 靶点名称 | Kappa Light Chain |
| 抗原 | Crude thymus membrane fraction. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 背景介绍 | Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30. |
| 研究领域 | Immune System antibody |
检测图片 (1) Click the Picture to Zoom In
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ARG63045 Mouse anti-Human Kappa Light Chain antibody [MEM-09] WB image
Western blot: Human blood serum samples of different healthy donors stained with ARG63045 Mouse anti-Human Kappa Light Chain antibody [MEM-09].
克隆号文献
Cell line-specific control of recombinant monoclonal antibody production by CHO cells.
WB / Chinese Hamster
Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen.
