ARG81837

Mouse Surfactant protein D ELISA Kit

Mouse Surfactant protein D ELISA 试剂盒 for ELISA and Mouse

内含物

货号 内含物名称 包装 温度
ARG81837-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81837-002 Standard 2 X 20 ng/vial 4°C
ARG81837-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81837-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81837-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81837-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81837-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81837-008 25X Wash buffer 20 ml 4°C
ARG81837-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81837-010 STOP solution 10 ml (Ready to use) 4°C
ARG81837-011 Plate sealer 4 strips Room temperature

概述

产品描述 ARG81837 Mouse Surfactant protein D ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Surfactant protein D in serum, plasma (heparin, EDTA) and cell culture supernatants.
反应物种 Ms
应用 ELISA
特异性 There is no detectable cross-reactivity with other relevant proteins.
靶点名称 Surfactant protein D
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm.
灵敏度 156 pg/ml
样品类型 Serum, plasma (heparin, EDTA) and cell culture supernatants.
标准范围 312.5 - 20000 pg/ml
样本量 100 µl
精确 Intra-Assay CV: 6.0%; Inter-Assay CV: 7.2%
別名 SP-D; COLEC7; Lung surfactant protein D; SFTP4; Pulmonary surfactant-associated protein D; PSP-D; Collectin-7

应用说明

检测时间 ~ 5 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 20390 Mouse SFTPD

Swiss-port # P50404 Mouse Pulmonary surfactant-associated protein D

基因名称 SFTPD
全名 surfactant protein D
背景介绍 The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015]
生物功能 Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. [UniProt]
产品亮点 Related products:
Surfactant protein D ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
翻译后修饰 The N-terminus is blocked.

Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.

S-nitrosylation at Cys-35 and Cys-40 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG81837 Mouse Surfactant protein D ELISA Kit standard curve image

    ARG81837 Mouse Surfactant protein D ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81837 Mouse Surfactant protein D ELISA Kit User's manual download icon Download